Lindberg, GabriellaSiegel, Spencer2024-08-302024-08-302024https://hdl.handle.net/1794/30006Organoids are in-vitro constructs designed to replicate in-vivo organs. Organoids have a wide range of potential use cases within science and medicine, but are not yet clinically relevant due to multiple factors. In order to address these factors, multiple engineering approaches have been derived to aid in organoids creation. One such approach is the creation of designer matrices, tailored and fabricated extracellular matrices for use in organoids. Designer matrices are commonly created through biofabrication techniques, techniques which use modern fabrication methods with biological components. However, the materials used in biofabrication must adhere to a strict set of design criteria. This project aims to develop a chemically modified form of alginate that can be used to form a hybrid hydrogel with allyl modified gelatin (Gel-AGE). This hydrogel would satisfy many of the biofabrication design criteria, and also contain a dual crosslinking system. Allylated Alginate (Al-AGE) was successfully synthesized, and was characterized via proton NMR. Al-AGE was tested for both ionic and covalent crosslinking abilities, and was shown to be able to undergo both forms of crosslinking. Finally, hybrid gels were assembled with Al-AGE and Gel-AGE.en-USCC BY-NC-ND 4.0Allylated AlginateBiofabricationBioengeneeringAl-AGESynthesisThesis/Dissertation0009-0001-5545-6502