Moslehi, SabaWatterson, William J.Rowland, ConorSmith, Julian H.Perez, Maria-TherezaTaylor, Richard P.2022-10-182022-10-182020-12-18Saba M, William J W, Conor R, Julian H. S, Maria-Thereza P, Richard P T. Physical Guidance of Cultured Retinal Neurons Using Zig-zag Surface Patterns. Am J Biomed Sci & Res. 2020 - 11(3). AJBSR.MS.ID.001629. DOI: 10.34297/AJBSR.2020.11.001629.doi.org/10.34297/AJBSR.2020.11.001629https://hdl.handle.net/1794/277093 pagesThe use of physical cues to control and guide various types of cells in vitro, especially neurons and their processes, has been the focus of a large amount of research. The response of neuronal processes to artificial surfaces depends on a number of factors including the cell type, the surface chemistry of the material, and the surface’s topological features [1,2]. In this Opinion piece, we investigate the extent to which retinal neuronal processes can be made to follow straight lines patterned into a surface. We show they can follow lines with relatively shallow heights of 2 μm and be made to undergo directional changes as great as 50°. However, some processes leave the lines and assume a weaving trajectory as they grow into the surface’s unpatterned regions. Based on these findings, we propose that neuronal processes will follow lines more closely if their shapes mimic the fractal weave patterns of unrestricted neurons. In addition to exploring the fundamental behavior of neurons interacting with artificial surfaces, the results inform the design of bio-inspired electrodes for human implants.enCreative Commons BY-NC-ND 4.0-USFractalIn VitroPhysical CuesRetinal NeuronsSu8Article