Data can be found here:  	http://lib-vm-rdmi.uoregon.edu/data/20164/

1.	Data File Name (including extension)

numerous file names -- see sub-folders


2.	Dataset name

Phase separated giant lipid vesicles. Raw image data are organized by lipid composition (see below) and by experiment date, with readme.txt files in each date's folder. Empty folders can be ignored, as can folders without readme.txt files (which largely consist of draft figures and other derived files). Analysis programs are collected in Hormel_Parthasarathy_Analysis_Programs.zip, which also contains a readme.txt file.

Lipid compositions are indicated in the folder names as follows:
  1-1 dopc-dppc 30 co: 1:1 DOPC:DPPC, 30% cholesterol, 1% Texas Red-DHPE
  2-1 dopc-dppc 20p co: 2:1 DOPC:DPPC, 20% cholesterol, 1% Texas Red-DHPE
  2-1 dppc-dopc 20p co: 1:2 DOPC:DPPC, 20% cholesterol, 1% Texas Red-DHPE
  2-1 dppc-dopc 40p co: 1:2 DOPC:DPPC, 40% cholesterol, 1% Texas Red-DHPE
  4-1 dopc-dppc 20p co: 4:1 DOPC:DPPC, 20% cholesterol, 1% Texas Red-DHPE
  9-1 dppc-dopc 30p co: 1:9 DOPC:DPPC, 30% cholesterol, 1% Texas Red-DHPE
  30 CO 25 DOPC 45 DPPC: 25:45:30 mol% DOPC:DPPC:cholesterol, 1% Texas Red-DHPE
  Low TR Control 2-1 dppc-dopc 40p C: 1:2 DOPC:DPPC, 40% cholesterol, 0.2% Texas Red-DHPE

3.	Other Files Related to These Data (Other Files in Set, e.g.)

n/a


4.	Author(s) Names (or person responsible for collecting the data):

Tristan Hormel
Matthew A. Reyer
Raghuveer Parthasarathy


5.	Author(s) ORCID IDs. 

    Prof. Raghuveer Parthasarathy
    ORCID: 0000-0002-6006-4749


6.	Contact Information

   Raghuveer Parthasarathy (Contact person)
   Department of Physics
   The University of Oregon
   Mail:
     Prof. Raghuveer Parthasarathy
     1274 University of Oregon
     Eugene, OR 97403-1274
   Email:
     raghu@uoregon.edu


7.	Date(s) of Data Collection 

2014-2015


8.	Date File was Created

August 18, 2016


9.	Date(s) and Nature of Updates

n/a


Basic Information- Data Use
10.	Citations of Publications Using these Data

Tristan T. Hormel, Matthew A. Reyer, Raghuveer Parthasarathy, "Two-Point Microrheology of Phase-Separated Domains in Lipid Bilayers." Biophys. J. 109: 732-726 (2015). [PMID 26287625] 

Abstract:
Though the importance of membrane fluidity for cellular function has been well established for decades, methods for measuring lipid bilayer viscosity remain challenging to devise and implement. Recently, approaches based on characterizing the Brownian dynamics of individual tracers such as colloidal particles or lipid domains have provided insights into bilayer viscosity. For fluids in general, however, methods based on single-particle trajectories provide a limited view of hydrodynamic response. The technique of two-point microrheology, in which correlations between the Brownian dynamics of pairs of tracers report on the properties of the intervening medium, characterizes viscosity at length-scales that are larger than that of individual tracers and has less sensitivity to tracer-induced distortions, but has never been applied to lipid membranes. We present, to our knowledge, the first two-point microrheological study of lipid bilayers, examining the correlated motion of domains in phase-separated lipid vesicles and comparing one- and two-point results. We measure two-point correlation functions in excellent agreement with the forms predicted by two-dimensional hydrodynamic models, analysis of which reveals a viscosity intermediate between those of the two lipid phases, indicative of global fluid properties rather than the viscosity of the local neighborhood of the tracer.


11.	Digital Object Identifiers (DOIs) of Publications Using these Data

DOI: http://dx.doi.org/10.1016/j.bpj.2015.07.017

12.	Purpose of Collection	

Fluorescence microscopy of the diffusion of phase-separated domains in lipid vesicles


Basic Information- Data Description
13.	Keywords and Definitions

lipid vesicles, phase separation, fluorescence microscopy, microrheology

14.	Example of Data in Set

n/a

15.	Lineage of Dataset

n/a

16.	Geographic Information

n/a

Methodological Information
17.	Method description, links or references to publications or other documentation containing experimental design or protocols used in data collection

See the published paper (item 10) for methods


18.	Any instrument-specific information needed to understand or interpret the data

n/a

19.	Standards and calibration information, if appropriate

n/a


20.	Describe any quality-assurance procedures performed on the data

n/a

21.	Definitions of codes or symbols used to note or characterize low quality/questionable/outliers that people should be aware of

n/a

22.	People involved with sample collection, processing, analysis and/or submission

see Authors

Data-specific Information



Sharing/Access information

Cite:
Tristan T. Hormel, Matthew A. Reyer, Raghuveer Parthasarathy, "Two-Point Microrheology of Phase-Separated Domains in Lipid Bilayers." Biophys. J. 109: 732-726 (2015). [PMID 26287625] 

Funding: 
National Science Foundation, Award No. 1006171

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.