Oregon Undergraduate Research Journal: Vol. 22 No. 1 (2024)

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Browsing Oregon Undergraduate Research Journal: Vol. 22 No. 1 (2024) by Subject "drosophila melanogaster"

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    Exploring the Role of Microbiota in the Development of Insulin-producing Cells in Drosophila melanogaster
    (University of Oregon, 2024) Mullen, Nicole
    Resident microbiota can influence many aspects of host health and disease. Research by the Guillemin lab demonstrates that gut microbiota induce the expansion of insulin-producing beta cells in zebrafish and mice via a bacterial protein, beta-cell expansion factor A (BefA). This study investigates microbiota, bacteria, and BefA protein roles in promoting insulin-producing cell (IPC) development in Drosophila melanogaster, where each brain lobe contains seven IPCs. The study evaluates the effect of germ-free (GF) rearing on IPC numbers in Drosophila and explores whether feeding flies BefA, utilizing transgenic BefA expression, or employing transgenic tsl, a known pore-forming protein, can restore IPC numbers in GF flies. The groups compared were GF, conventionally reared (CV), GF flies fed BefA, and GF flies with transgenic BefA or tsl expression. Tissue-specific Dilp3:GAL4/UAS:GFP and immunohistochemistry treatment enables IPC visualization post-dissection. Findings show fewer IPCs per brain lobe in GF flies compared to conventional, highlighting microbiota's role in IPC development. GF larvae fed BefA exhibit a slight but significant IPC increase per lobe versus conventional, suggesting BefA's potential to mitigate GF effects. Transgenic expression of BefA, using the GAL4/UAS system, demonstrates a trending IPC increase in GF flies, while transgenic tsl expression significantly increases IPCs. These results indicate microbiota's impact on Drosophila metabolic pathways and fundamental cell development, including cells in the gut-brain axis. These insights can be used to direct research and treatment for diseases like diabetes and have implications for microbiota's effect on the brain. Future experiments will explore BefA's properties, including its potential to induce insulin-producing cell expansion through membrane permeabilization.