Analyzing Early Embryonic Cell Division Defects in Caenorhabditis Elegans

Simple item page
dc.contributor.authorKrishnakumar, Sheetal
dc.date.accessioned2014-09-18T17:58:58Z
dc.date.available2014-09-18T17:58:58Z
dc.date.issued2014-06
dc.description36 pages. A thesis presented to the Department of Biology and the Clark Honors College of the University of Oregon in partial fulfillment of the requirements for degree of Bachelor of Science, Spring 2014.en_US
dc.description.abstractSearching for and analyzing novel mutant phenotypes in early cell division of C. elegans allows researcher to infer how the wild-type gene/ protein normally functions after observing defects. To investigate these phenotypes, three different temperature sensitive (ts) mutants (orl786ts; lin-2 (el309), orl700ts; /iu-2 (el309), and orl578ts; Jin-2 (el309)) were analyzed. Using Nomarsk.i/ DIC videomicroscopy, phenotypic analysis was completed to record the mutant embryos from the 1-cell to 4-cell embryo stage. After following up the phenotypic analysis with outcrosses and re-analysis, the mutants showed to have several defects prolonged durations within stages of the cell cycle. This could be a result of mutations in DNA replication machinery or of cell cycle regulator. Further tests can be done to isolate the origin of the mutation and permit a better understanding of the genes in which the mutations occurred.en_US
dc.identifier.urihttps://hdl.handle.net/1794/18252
dc.language.isoen_USen_US
dc.publisherUniversity of Oregonen_US
dc.relation.ispartofseriesUniversity of Oregon thesis, Dept. of Biology, Honors College, B.S., 2014;
dc.rightsAll Rights Reserved.en_US
dc.subjectC. elegansen_US
dc.subjectCell divisionen_US
dc.subjectEarly embryonicen_US
dc.subjectCaenorhabditis elegansen_US
dc.subjectBiologyen_US
dc.titleAnalyzing Early Embryonic Cell Division Defects in Caenorhabditis Elegansen_US
dc.typeThesis / Dissertationen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Thesis Final-Krishnakumar.pdf
Size:
1.12 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
Name:
license.txt
Size:
2.13 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Clark Honors College Theses