Microregulation of zebrafish skeletal development by microRNAs
| dc.contributor.author | He, Xinjun, 1982- | |
| dc.date.accessioned | 2011-05-25T18:19:06Z | |
| dc.date.available | 2011-05-25T18:19:06Z | |
| dc.date.issued | 2010-09 | |
| dc.description | xvii, 125 p. : ill. (some col.) A print copy of this thesis is available through the UO Libraries. Search the library catalog for the location and call number. | en_US |
| dc.description.abstract | MicroRNAs are small regulatory RNAs that control various developmental and physiological processes in animals and plants. To study the involvement of microRNAs in skeletal development, I manipulated the expression of miR-140, which is strongly expressed in the developing skeleton, and miR-196, which is located among the body patterning Hox cluster genes. I found that miR-140 regulates zebrafish palate formation by interfering with neural crest cell migration through the inhibition of the expression of the platelet derived growth factor receptor alpha ( pdgfra ) gene. I also found that miR196 regulates zebrafish pectoral fin initiation by regulating the expression of the retinoic acid receptor alpha b ( rarab ) gene and that miR-196 is involved in the patterning of zebrafish pharyngeal arches and vertebrae. These results illuminate previously unknown regulatory mechanisms of skeletal development. I also reviewed current knowledge concerning microRNAs in skeletal development and evolution and discussed potential relationships between microRNAs and skeletal disease. This dissertation includes previously published and unpublished coauthored material. | en_US |
| dc.description.sponsorship | Committee in charge: Judith Eisen, Chairperson, Biology; John Postlethwait, Advisor, Biology; Charles Kimmel, Member, Biology; William Cresko, Member, Biology; J. Andrew Berglund, Outside Member, Chemistry | en_US |
| dc.identifier.uri | https://hdl.handle.net/1794/11187 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | University of Oregon | en_US |
| dc.relation.ispartofseries | University of Oregon theses, Dept. of Biology, Ph. D., 2010; | |
| dc.subject | Microregulation | en_US |
| dc.subject | Zebrafish | en_US |
| dc.subject | Skeletal development | en_US |
| dc.subject | MicroRNAs | en_US |
| dc.subject | Skeletogenesis | en_US |
| dc.subject | Genetics | en_US |
| dc.subject | Developmental biology | en_US |
| dc.title | Microregulation of zebrafish skeletal development by microRNAs | en_US |
| dc.type | Thesis | en_US |
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