Chromatin Regulation: How Nucleosomes Find their Positions and the Role of Chromatin Binding Proteins in Cellular Quiescence

Simple item page
dc.contributor.advisorSelker, Eric
dc.contributor.authorBailey, Thomas
dc.date.accessioned2023-03-24T18:56:36Z
dc.date.available2023-03-24T18:56:36Z
dc.date.issued2023-03-24
dc.description.abstractChromatin is made up of an organism’s DNA and DNA binding proteins inside of the nucleus. Nucleosomes are the fundamental unit of chromatin, consisting of a 147 base pair DNA sequence and a protein octamer comprised of 8 histone proteins, two copies of histone H2A, H2B, H3, and H4. Nucleosomes are used to regulate gene expression in both the positioning of the nucleosome along DNA, or the post-translational histone modifications. In the first chapter, we will demonstrate that the chromatin binding protein, Tup1 is essential for nucleosome positioning and H3K23 deacetylation, which is required for entry into cellular quiescence. In the next chapter, we will address how the chromatin remodeler complex, Isw2, precisely positions nucleosomes through direct interactions with transcription factors. In the next chapter, we present an optimized protocol for measuring nucleosome positioning. Finally, we will end on some preliminary data and discussion on the next steps in researching Tup1 and Isw2.This dissertation contains previously published co-authored material.en_US
dc.identifier.urihttps://hdl.handle.net/1794/28090
dc.language.isoen_US
dc.publisherUniversity of Oregon
dc.rightsAll Rights Reserved.
dc.subjectCell Cycleen_US
dc.subjectChromatin Remodelingen_US
dc.subjectEpigeneticsen_US
dc.subjectIsw2en_US
dc.subjectQuiescenceen_US
dc.subjectTup1en_US
dc.titleChromatin Regulation: How Nucleosomes Find their Positions and the Role of Chromatin Binding Proteins in Cellular Quiescence
dc.typeElectronic Thesis or Dissertation
thesis.degree.disciplineDepartment of Chemistry and Biochemistry
thesis.degree.grantorUniversity of Oregon
thesis.degree.leveldoctoral
thesis.degree.namePh.D.

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Bailey_oregon_0171A_13484.pdf
Size:
2.15 MB
Format:
Adobe Portable Document Format
Download

Collections

Theses and Dissertations
Chemistry Theses and Dissertations