The Genetic Basis of Natural Variation in the Response to Adult Starvation in Caenorhabditis elegans
| dc.contributor.advisor | Phillips, Patrick | |
| dc.contributor.author | Archer, Heather | |
| dc.date.accessioned | 2019-09-18T19:29:38Z | |
| dc.date.available | 2019-09-18T19:29:38Z | |
| dc.date.issued | 2019-09-18 | |
| dc.description.abstract | Caenorhabditis elegans typically feeds on rotting fruit and plant material in a fluctuating natural habitat, a boom-and-bust lifestyle. Moreover, stage specific developmental responses to low food concentration suggest that starvation-like conditions are a regular occurrence. In order to assess variation in the C. elegans starvation response under precisely controlled conditions and simultaneously phenotype a large number of individuals with high precision, we have developed a microfluidic device that, when combined with image scanning technology, allows for high-throughput assessment at a temporal resolution not previously feasible and applied this to a large mapping panel of fully sequenced intercross lines. Under these conditions worms exhibit a markedly reduced adult lifespan with strain-dependent variation in starvation resistance, ranging from <24 hours to ~120 hours. Genome-wide mapping of the responses of more than 7,855 individuals suggests four loci of large effects. Three of these loci are associated with single genes (ash-2, exc-6, and dpy-28) and the fourth is a ~26 KB region on Chromosome V encompassing several genes. Backcross with selection confirmed the effect of the Chromosome V locus. Overall, there is a clear genetic basis for natural variation in the response to food availability within this species. | en_US |
| dc.identifier.uri | https://hdl.handle.net/1794/24930 | |
| dc.language.iso | en_US | |
| dc.publisher | University of Oregon | |
| dc.rights | All Rights Reserved. | |
| dc.subject | diapause | en_US |
| dc.subject | elegans | en_US |
| dc.subject | evolution | en_US |
| dc.subject | genetics | en_US |
| dc.subject | microfluidics | en_US |
| dc.subject | starvation | en_US |
| dc.title | The Genetic Basis of Natural Variation in the Response to Adult Starvation in Caenorhabditis elegans | |
| dc.type | Electronic Thesis or Dissertation | |
| thesis.degree.discipline | Department of Biology | |
| thesis.degree.grantor | University of Oregon | |
| thesis.degree.level | doctoral | |
| thesis.degree.name | Ph.D. |
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