Investigation of Pore-Forming Transmembrane Toxins using Native Ion-Mobility Mass Spectrometry

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Date

2017

Authors

Young, Isabella

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Abstract

Antrhax toxin (ATX) and Alpha-Hemolysin (AHL) are examples of large transmembrane pore-forming toxins that are similar in structure and are proposed to have specific protein-lipin interactions. Due to the difficulty of studying these structures in solution, native mass spectrometry (native-MS) was used to examine the structure, stoichiometry, and lipid-binding of these membrane protein complexes and ion mobility (IM) analysis was used to further study the complexes' shape and size.

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1 page poster

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