Design and Cytocompatibility of Hyaluronic Acid Hydrogels for Bone Regeneration
| dc.contributor.advisor | Hettiaratchi, Marian | |
| dc.contributor.author | Johnson, Ireland | |
| dc.contributor.author | Guerrero, Andres | |
| dc.contributor.author | Spaulding, Veronica | |
| dc.contributor.author | Hettiaratchi, Marian | |
| dc.date.accessioned | 2021-07-27T16:49:49Z | |
| dc.date.available | 2021-07-27T16:49:49Z | |
| dc.date.issued | 2021 | |
| dc.description | 1 page. | |
| dc.description.abstract | Large bone defects and fractures caused by trauma or disease remain a serious challenge for orthopedic surgeons, and there is a need for more effective treatment strategies to repair injured bone. Bone autografts, a tissue graft from the same patient, are the ideal material to promote a healing response due to low host rejection; however, they can lead to donor site morbidity and are expensive to extract. To combat this problem, biomaterials, composed of the natural polymer hyaluronic acid (HA) can be used to deliver osteogenic (bone-forming) proteins that repair injured bone. This study describes the development of HA-based hydrogels for protein delivery for bone regeneration. HA hydrogels were formed by dynamic, covalent bonds between aldehyde functional groups on oxidized HA and HA functionalized with adipic acid hydrazide or carbohydrazide groups. Hydrogels were seeded with 3T3 fibroblast cells expressing green fluorescent protein to evaluate cell compatibility. Live and dead cells were evaluated using green fluorescence from GFP and red fluorescence from ethidium homodimer, respectively. A combination of oxidized HA and HA-carbohydrazide at 2.5% (w/v) maintained high cell viability (>82.3% for all time points) and encouraged a rate of cell growth that surpassed all other conditions. Future expansions of this project could lead to the use of HA hydrogels as a biomaterial that rivals the healing response of bone autografts. | en_US |
| dc.format.mimetype | application/pdf | |
| dc.identifier.orcid | 0000-0002-5223-3426 | |
| dc.identifier.uri | https://hdl.handle.net/1794/26425 | |
| dc.language.iso | en_US | |
| dc.publisher | University of Oregon | |
| dc.rights | CC BY-NC-ND 4.0 | |
| dc.subject | Hydrogels | en_US |
| dc.subject | Bone Regeneration | en_US |
| dc.subject | Hyaluronic Acid | en_US |
| dc.title | Design and Cytocompatibility of Hyaluronic Acid Hydrogels for Bone Regeneration | |
| dc.type | Presentation |