Effects of Molting and Hyposalinity Stress on the Expression of HIF-a, Molting, and Immune Response Genes in Juvenile Cancer magister
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Date
2009-03
Authors
Miller, Wyatt, 1980-
Journal Title
Journal ISSN
Volume Title
Publisher
University of Oregon
Abstract
Molting and hyposalinity stress in crustaceans cause extensive morphological and
metabolic changes. I hypothesize that injuvenile Cancer magister, which molt in
nearshore and estuarine habitats, hypoxia inducible factor (HIF) participates in regulating
target genes in response to molting and hyposalinity stress. This study investigated the
mRNA expression ofHIF-a, cryptocyanin 2, arthrodial membrane protein 6.0, anti..
lipopolysaccharide factor (ALF), and p-actin in juvenile C. magister under normoxic
conditions. One cohort of 1st instar juveniles was maintained across the entire molt
cycle. Beginning at ecdysis and daily until 2nd instar, crabs were sampled for total RNA
and analyzed by real-time quantitative PCR. A second cohort was exposed to 50%
seawater for 3, 8, and 24 hours and then analyzed for mRNA expression. All five genes showed molt stage-specific changes in mRNA expression during the molt
cycle in normoxia, but the genes did not change expression due to hyposalinity stress.
Description
xi, 49 p. : ill. A print copy of this thesis is available through the UO Libraries. Search the library catalog for the location and call number.