dc.contributor.author |
He, Xinjun, 1982- |
|
dc.date.accessioned |
2011-05-25T18:19:06Z |
|
dc.date.available |
2011-05-25T18:19:06Z |
|
dc.date.issued |
2010-09 |
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dc.identifier.uri |
http://hdl.handle.net/1794/11187 |
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dc.description |
xvii, 125 p. : ill. (some col.) A print copy of this thesis is available through the UO Libraries. Search the library catalog for the location and call number. |
en_US |
dc.description.abstract |
MicroRNAs are small regulatory RNAs that control various developmental and physiological processes in animals and plants. To study the involvement of microRNAs in skeletal development, I manipulated the expression of miR-140, which is strongly expressed in the developing skeleton, and miR-196, which is located among the body patterning Hox cluster genes. I found that miR-140 regulates zebrafish palate formation by interfering with neural crest cell migration through the inhibition of the expression of the platelet derived growth factor receptor alpha ( pdgfra ) gene. I also found that miR196 regulates zebrafish pectoral fin initiation by regulating the expression of the retinoic acid receptor alpha b ( rarab ) gene and that miR-196 is involved in the patterning of zebrafish pharyngeal arches and vertebrae. These results illuminate previously unknown regulatory mechanisms of skeletal development. I also reviewed current knowledge concerning microRNAs in skeletal development and evolution and discussed potential relationships between microRNAs and skeletal disease.
This dissertation includes previously published and unpublished coauthored material. |
en_US |
dc.description.sponsorship |
Committee in charge: Judith Eisen, Chairperson, Biology;
John Postlethwait, Advisor, Biology;
Charles Kimmel, Member, Biology;
William Cresko, Member, Biology;
J. Andrew Berglund, Outside Member, Chemistry |
en_US |
dc.language.iso |
en_US |
en_US |
dc.publisher |
University of Oregon |
en_US |
dc.relation.ispartofseries |
University of Oregon theses, Dept. of Biology, Ph. D., 2010; |
|
dc.subject |
Microregulation |
en_US |
dc.subject |
Zebrafish |
en_US |
dc.subject |
Skeletal development |
en_US |
dc.subject |
MicroRNAs |
en_US |
dc.subject |
Skeletogenesis |
en_US |
dc.subject |
Genetics |
en_US |
dc.subject |
Developmental biology |
en_US |
dc.title |
Microregulation of zebrafish skeletal development by microRNAs |
en_US |
dc.type |
Thesis |
en_US |