Xylaria Primers for Phylogenetic Reconstruction
Datum
2015-06
Autor:innen
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Verlag
University of Oregon
Zusammenfassung
Xylaria is a genus of Ascomycete fungi that is comprised of an estimated 400 species.
These species are important to the ecosystems in which they inhabit, because they contribute to
the decomposition of wood, which recycles lignin and cellulose. Though this genus’s
morphological traits have been widely studied over the years, their molecular phylogenetics have
not been extensively explored due to the historical unavailability of species genomes. Now that
complete genomes have become available, there have been an increasing number of ribosomal
Internal Transcribed Spacer (ITS) sequence studies in recent years. However, ITS is too variable
to consistently construct accurate phylogenetic trees, so Xylaria phylogeny is still being
researched. This study explores the use of single-copy genes as an alternative to ITS methods.
Here I present three primer pairs for different single-copy genes for use during future
phylogenetic evaluation of Xylaria. These primers, Splicing Factor PRP43 F1054/R1691, MCM5
F409/R1059 and 40S Ripro S3Ae F47/R605, are more reliable than ITS sequences for deeper
levels of analysis. This study focuses on Xylaria, while utilizing related species within in the
Xylariaceae and Sordariaceae families, providing a promising start to the complete
reconstruction of the Xylaria phylogenetic tree.
Beschreibung
33 pages
Schlagwörter
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