Effects of Molting and Hyposalinity Stress on the Expression of HIF-a, Molting, and Immune Response Genes in Juvenile Cancer magister
Miller, Wyatt, 1980-
Molting and hyposalinity stress in crustaceans cause extensive morphological and metabolic changes. I hypothesize that injuvenile Cancer magister, which molt in nearshore and estuarine habitats, hypoxia inducible factor (HIF) participates in regulating target genes in response to molting and hyposalinity stress. This study investigated the mRNA expression ofHIF-a, cryptocyanin 2, arthrodial membrane protein 6.0, anti.. lipopolysaccharide factor (ALF), and p-actin in juvenile C. magister under normoxic conditions. One cohort of 1st instar juveniles was maintained across the entire molt cycle. Beginning at ecdysis and daily until 2nd instar, crabs were sampled for total RNA and analyzed by real-time quantitative PCR. A second cohort was exposed to 50% seawater for 3, 8, and 24 hours and then analyzed for mRNA expression. All five genes showed molt stage-specific changes in mRNA expression during the molt cycle in normoxia, but the genes did not change expression due to hyposalinity stress.