Analyzing Early Embryonic Cell Division Defects in Caenorhabditis Elegans
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Date
2014-06
Authors
Krishnakumar, Sheetal
Journal Title
Journal ISSN
Volume Title
Publisher
University of Oregon
Abstract
Searching for and analyzing novel mutant phenotypes in early cell division of C.
elegans allows researcher to infer how the wild-type gene/ protein normally functions
after observing defects. To investigate these phenotypes, three different temperature
sensitive (ts) mutants (orl786ts; lin-2 (el309), orl700ts; /iu-2 (el309), and orl578ts;
Jin-2 (el309)) were analyzed. Using Nomarsk.i/ DIC videomicroscopy, phenotypic
analysis was completed to record the mutant embryos from the 1-cell to 4-cell embryo
stage. After following up the phenotypic analysis with outcrosses and re-analysis, the
mutants showed to have several defects prolonged durations within stages of the cell
cycle. This could be a result of mutations in DNA replication machinery or of cell cycle
regulator. Further tests can be done to isolate the origin of the mutation and permit a
better understanding of the genes in which the mutations occurred.
Description
36 pages. A thesis presented to the Department of Biology and the Clark Honors College of the University of Oregon in partial fulfillment of the requirements for degree of Bachelor of Science, Spring 2014.
Keywords
C. elegans, Cell division, Early embryonic, Caenorhabditis elegans, Biology