Propagating Putative Prion States in Budding Yeast

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Date

2020

Authors

Evarts, Jacob
Garcia, David

Journal Title

Journal ISSN

Volume Title

Publisher

University of Oregon

Abstract

Prions have long been closely associated with fatal neurodegenerative diseases. Recent evidence suggests that prions provide an additional class of epigenetic mechanism that works at a rapid pace. From an evolutionary standpoint, the ability to change phenotypes without waiting for a genetic variance would be hugely beneficial in a high-stress environment. Using two known techniques for increasing de novo prion formation, protein overexpression, and environmental stressor, the Garcia Lab performed a large-scale screen across many RNA modifying enzymes in budding yeast. The initial analysis of the resulting growth curves suggests that putative prion-state induction could be a widespread epigenetic mechanism across yeast.

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Keywords

Budding Yeast, Prions, RNA, Proteins, Computational Biology

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