Biology Faculty Research

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Browsing Biology Faculty Research by Author "Villeneuve, Anne M."

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    Epitope tag-specific differences in the detection of COSA-1 marked crossover sites in C. elegans spermatocytes
    (Caltech Library, 2023-01-06) Cahoon, Cori K.; Uebel, Celja J.; Villeneuve, Anne M.; Libuda, Diana E.
    Nascent crossover sites in C. elegans meiocytes can be cytologically detected using epitope-tagged versions of the pro-crossover protein COSA-1. In spermatocytes, differences exist between cytologically-detected and genetically-detected double crossover rates. Here, we examine nascent crossovers using both GFP- and OLLAS-tagged COSA-1. Similar to previous work, we find that most late pachytene spermatocytes display 5 COSA-1 foci, indicating one crossover per autosome bivalent. However, we detected more nuclei with >5 COSA-1 foci using OLLAS::COSA-1, reflecting some bivalents having 2 COSA-1 foci. These results demonstrate tag-specific differences in the detection of COSA-1 marked nascent crossovers in spermatocytes.