Epitope tag-specific differences in the detection of COSA-1 marked crossover sites in C. elegans spermatocytes
Loading...
Date
2023-01-06
Authors
Cahoon, Cori K.
Uebel, Celja J.
Villeneuve, Anne M.
Libuda, Diana E.
Journal Title
Journal ISSN
Volume Title
Publisher
Caltech Library
Abstract
Nascent crossover sites in C. elegans meiocytes can be cytologically detected using epitope-tagged versions of the pro-crossover protein COSA-1. In spermatocytes, differences exist between cytologically-detected and genetically-detected double crossover rates. Here, we examine nascent crossovers using both GFP- and OLLAS-tagged COSA-1. Similar to previous work, we find that most late pachytene spermatocytes display 5 COSA-1 foci, indicating one crossover per autosome bivalent. However, we detected more nuclei with >5 COSA-1 foci using OLLAS::COSA-1, reflecting some bivalents having 2 COSA-1 foci. These results demonstrate tag-specific differences in the detection of COSA-1 marked nascent crossovers in spermatocytes.
Description
6 jpages
Keywords
Citation
Cahoon, CK; Uebel, CJ; Villeneuve, AM; Libuda, DE (2023). Epitope tag-specific differences in the detection of COSA-1 marked crossover sites in C. elegans spermatocytes. microPublication Biology. 10.17912/micropub.biology.000724.