A high vacuum photoelectron microscope for the study of biological specimens
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Date
1981
Authors
Griffith, O. H.
Rempfer, G. F.
Lesch, G. H.
Journal Title
Journal ISSN
Volume Title
Publisher
Scanning Electron Microscopy
Abstract
A photoelectron microscope (photoemission
electron microscope) has been designed and built
for the study of organic and biological samples.
The microscope is an oil-free stainless steel high
vacuum instrument pumped by a titanium sublimation
pump, an ion pump, and molecular sieve roughing
pumps. The electron lenses are of the electrostatic
unipotential type. The microscope is
equipped with a dewar for sample cooling, an
internal cryogenic camera, TV-image intensifier,
and vibration isolation support. Applications
include studies of biological cell surfaces, photosynthetic
membranes and aromatic chemical
carcinogens. A representative micrograph of mouse
3T3 cells is included. In some respects, photoelectron
micrographs resemble scanning electron
micrographs, but the basis for contrast is
different in these two techniques.
Description
8 pages
Keywords
Citation
Griffith, O. H., Rempfer, G. F. & Lesch, G. H. (1981) A high vacuum photoelectron microscope for the study of biological specimens. Scanning Electron Microsc., pp 123-130, SEM Inc., AMF O'Hare, Chicago, IL.