Immunophotoelectron Microscopy: The Electron Optical Analog of Immunofluorescence Microscopy
| dc.contributor.author | Birrell, G. B. | |
| dc.contributor.author | Habliston, D. L. | |
| dc.contributor.author | Nadakavukaren, K. K. | |
| dc.contributor.author | Griffith, O. H. | |
| dc.date.accessioned | 2016-05-27T19:14:08Z | |
| dc.date.available | 2016-05-27T19:14:08Z | |
| dc.date.issued | 1985-01 | |
| dc.description | 5 pages | en_US |
| dc.description.abstract | The electron optical analog of immunofluo rescence microscopy combines three developments: (i) photoelectron microscopy to produce a high-resolution image of ex posed components of the cell, (jQ site-specific antibodies, and (iii) photoemissive markers coupled to the antibodies to make the distribution of sites visible. This approach, in theory, pro vides a way to extend the useful immunofluorescence micros copy technique to problems requiring much higher resolution. The resolution limit of fluorescence microscopy is limited to about 200 nm by the wavelength of the light used to form the image, whereas in photoelectron microscopy the image is formed by electrons (current resolution: 10-20 nm; theoretical limit: 5 nm or better depending on the electron optics). As a test system, cytoskeletons of CV-1 epithelial cells were pre pared under conditions that preserve microtubules, and the microtubule networks were visualized by both indirect immu nofluorescence and immunophotoelectron microscopy using colloidal gold coated with antibodies. Colloidal gold serves as a label for immunophotoelectron microscopy, providing en hanced photoemission from labeled cellular components so that they stand out against the darker background of the re maining unlabeled structures. In samples prepared for both immunofluorescence and immunophotoelectron microscopy, individual microtubules in the same cells were visualized by both techniques. The photoemissionof the colloidal gold mark ers is sufficiently high that the microtubules are easily recog nized without reference to the immunofluorescence micro graphs, indicating that this approach can be used, in combina tion with antibodies, to correlate structure and function in cell biological studies. | en_US |
| dc.identifier.citation | Birrell, G. B. , Habliston, D. L., Nadakavukaren, K. K. & Griffith, O. H. (1985) Immunophotoelectron Microscopy: The Electron Optical Analog of Immunofluorescence Microscopy. Proc. Natl. Acad. Sci. USA, 82, 109‑113. | en_US |
| dc.identifier.uri | https://hdl.handle.net/1794/19900 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Proceedings of the National Academy of Sciences | en_US |
| dc.rights | Creative Commons BY-NC-ND 4.0-US | en_US |
| dc.title | Immunophotoelectron Microscopy: The Electron Optical Analog of Immunofluorescence Microscopy | en_US |
| dc.type | Article | en_US |