The Role of Chromatin Remodelers for Long-term Survival of Saccharomyces Cerevisiae in the Quiescent Phase

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dc.contributor.authorPledger, Erica Suzuki
dc.date.accessioned2019-11-07T16:14:26Z
dc.date.available2019-11-07T16:14:26Z
dc.date.issued2019
dc.description27 pages
dc.description.abstractChromatin remodelers are a group of enzymes that catalyze the movement of nucleosomes in order to allow other biomolecules access to DNA during processes such as transcription, replication, and DNA repair. Previous studies revealed that Chd1 and Isw1 chromatin remodelers in Saccharomyces cerevisiae are necessary for establishing regular nucleosome organization. In the absence of Chd1 and Isw1, a regular pattern of nucleosome positioning is abolished. However, the loss of nucleosome organization has surprisingly little effect on genome-wide transcription or on viability. Here, we test the hypothesis that nucleosome organization may have a more vital function during processes that require global alterations in transcription, such as quiescence. Yeast cells respond to low nutrient conditions by entering the quiescent phase, during which they exhibit significant decreases in transcriptional, translational, and metabolic activity. We isolated quiescent cells of chromatin remodeler knockout strains and monitored their survival in nutrient-starved conditions. Our results indicate that long-term viability in the quiescent phase is significantly reduced in strains lacking Chd1, Isw1, and Isw2, thus suggesting a role for chromatin remodelers during quiescence.en_US
dc.identifier.urihttps://hdl.handle.net/1794/25051
dc.language.isoen_US
dc.publisherUniversity of Oregon
dc.rightsCreative Commons BY-NC-ND 4.0-US
dc.subjectBiochemistryen_US
dc.subjectQulescenceen_US
dc.subjectChromatinen_US
dc.subjectSaccharomyces Cerevisiaeen_US
dc.subjectNucleosomeen_US
dc.subjectBiochemistryen_US
dc.titleThe Role of Chromatin Remodelers for Long-term Survival of Saccharomyces Cerevisiae in the Quiescent Phase
dc.typeThesis/Dissertation

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