Photoelectron microscopy of biological surfaces. Excitation source brightness requirements
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Date
1976
Authors
Dam, R. J.
Griffith, O. H.
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Photo-Optical Instrumentation Engineers
Abstract
Photoelectron microscopy is a surface technique which provides topographical infor
mation using the photoelectric effect as a basis for contrast. Progress in the biolo
gical applications of this technique is briefly reviewed. Due to relatively low quantum
yields, photoemission from biological samples is weak and an image intensifier is used
in order to visualize and record the photoelectron image. Currently the limiting
magnification is determined by UV power incident on the sample. Power requirements
for high-magnification imaging are calculated in terms of microscope, sample, and image
intensifier parameters. To approach 40 A resolution, an instrument magnification of
12,000-50,000 is required along with a UV intensity of 0.01 to 10 Watts/cm2 depending
on the wavelength and sample. For a tightly focused laser source the total power re
quirement is 1 mWatt or less.
Description
7 pages
Keywords
Citation
Dam, R. J. & Griffith, O. H. (1976) Photoelectron microscopy of biological surfaces. Excitation source brightness requirements. Soc. Photo‑Optical Instr. Engineers 78, 143‑154.