Evidence for boundary lipid in membranes

dc.contributor.authorJost, P. C.
dc.contributor.authorGriffith, O. H.
dc.contributor.authorCapaldi, R. A.
dc.contributor.authorVanderkooi, G.
dc.date.accessioned2016-05-25T22:50:11Z
dc.date.available2016-05-25T22:50:11Z
dc.date.issued1973-02
dc.description6 pagesen_US
dc.description.abstractABSTH\OT (Mochromc oxidase (EC 1.9.3.1) isolated from hccf-hcarl mitochondria with an appropriate phospholipid content forms vesicular structures. Lipid-protein interactions in this model membrane system were studied with the,lipid spin label, 16-doxylstearic acid. As the phospholipid/ prolein ratio is varied, two spectral components are ohserved. Al low phospholipid/prolcin ratios (<0.19 mg of phospholipid per mg of protein) the lipid spin label is liifCll!) immobilized. At higher phospholipid content an additional component characteristic of fluid lipid hilayers is evideiil. By summation of digilalized spectra and subsequent integration it was shown that all composite spec tra could he approximated by assuming only two com ponents arc present, and that the amount of phospholipid hound In Ihr protein is independent of the extent of the fluid hilaw-r region. The experimentally determined amount of phospholipid for maximum occupancy of pro- Iciii-hound niies is ahouI 0.2 mg of phospholipid per 1.0 nig itl protein. Iialiulalions show that this ralio is con sistent with a single layerof phospholipid surrounding the protein complex. The data are interpreted as evidence for a boundary of immobilized lipid between the hydrophobic protein and adjacent fluid hilayer regions in this membrane model system.en_US
dc.identifier.citationJost, P. C., Griffith, O. H., Capaldi, R. A. & Vanderkooi, G. (1973) Evidence for boundary lipid in membranes. Proc. Natl. Acad. Sci. USA 70, 480‑484.en_US
dc.identifier.urihttps://hdl.handle.net/1794/19888
dc.language.isoen_USen_US
dc.publisherProceedings of the National Academy of Sciencesen_US
dc.rightsCreative Commons BY-NC-ND 4.0-USen_US
dc.titleEvidence for boundary lipid in membranesen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Griffith_39.pdf
Size:
8.53 MB
Format:
Adobe Portable Document Format
Description:
License bundle
Now showing 1 - 1 of 1
Name:
license.txt
Size:
2.23 KB
Format:
Item-specific license agreed upon to submission
Description: