THE ISOLATION AND CHARACTERIZATION OF NOVEL POSTSYNAPTIC ANTIBODIES FOR DANIO RERIO

Show simple item record

dc.contributor.author Kou, Yuqing
dc.date.accessioned 2012-07-09T18:58:25Z
dc.date.available 2012-07-09T18:58:25Z
dc.date.issued 2012-05
dc.identifier.uri http://hdl.handle.net/1794/12243
dc.description 67 pages. A THESIS Presented to the Department of Chemistry and Biochemistry and the Clark Honors College of the University of Oregon in partial fulfillment of the requirements for degree of Bachelor of Science, Spring 2012. en_US
dc.description.abstract The proteins and molecular agents that govern synapse formation in developing organisms are essential for cognitive and neural maturation. Mutations or disruptions in proteins that aid in the formation of a mature synapse have been implicated in developmental and neural disorders such as autism, mental retardation, and schizophrenia. The current body of knowledge on synapses lacks great detail about the processes that occur following the release of neurotransmitters into the synapse, including the events occurring in the synaptic cleft and postsynaptically. The general aim of this project was to generate antibodies that would specifically recognize synaptic targets in order to better characterize the various processes of synaptogenesis, including the establishment of the postsynaptic apparatus and the recruitment of assorted structural proteins and neurotransmitter receptors. Clonal hybridomas were generated by injecting mice with purified fractions of synaptoneurosomes and postsynaptic density. A total of 96 clonal hybridomas were screened for the desired characteristics by enzyme-linked immunosorbent assay, immunolabeling, Western blotting, and immunoprecipitation. A number of hybridomas recognized targets with high affinity and exhibited specificity for neuronal and postsynaptic targets. Notably, a number of subclonal hybridomas labeled neuronal targets strongly in whole-mount immunolabeling. Additionally, immunoprecipitation showed that selected subclonal hybridomas could precipitate antigens from solution, which will allow the isolated antigens to be identified. en_US
dc.language.iso en_US en_US
dc.publisher University of Oregon en_US
dc.relation.ispartofseries University of Oregon theses, Dept. of Chemistry and Biochemistry, Honors College, B.S., 2012;
dc.rights rights_reserved en_US
dc.subject zebrafish en_US
dc.subject postsynaptic density en_US
dc.subject synapse formation en_US
dc.subject hybridoma en_US
dc.subject monoclonal antibody en_US
dc.subject immunolabeling en_US
dc.title THE ISOLATION AND CHARACTERIZATION OF NOVEL POSTSYNAPTIC ANTIBODIES FOR DANIO RERIO en_US
dc.type Thesis en_US


Files in this item

Files Size Format View Description
YKou_Thesis.pdf 2.304Mb PDF View/Open
kou_license.pdf 37.31Kb PDF View/Open license

This item appears in the following Collection(s)

Show simple item record