Epitope tag-specific differences in the detection of COSA-1 marked crossover sites in C. elegans spermatocytes
| dc.contributor.author | Cahoon, Cori K. | |
| dc.contributor.author | Uebel, Celja J. | |
| dc.contributor.author | Villeneuve, Anne M. | |
| dc.contributor.author | Libuda, Diana E. | |
| dc.date.accessioned | 2023-01-09T22:05:08Z | |
| dc.date.available | 2023-01-09T22:05:08Z | |
| dc.date.issued | 2023-01-06 | |
| dc.description | 6 jpages | en_US |
| dc.description.abstract | Nascent crossover sites in C. elegans meiocytes can be cytologically detected using epitope-tagged versions of the pro-crossover protein COSA-1. In spermatocytes, differences exist between cytologically-detected and genetically-detected double crossover rates. Here, we examine nascent crossovers using both GFP- and OLLAS-tagged COSA-1. Similar to previous work, we find that most late pachytene spermatocytes display 5 COSA-1 foci, indicating one crossover per autosome bivalent. However, we detected more nuclei with >5 COSA-1 foci using OLLAS::COSA-1, reflecting some bivalents having 2 COSA-1 foci. These results demonstrate tag-specific differences in the detection of COSA-1 marked nascent crossovers in spermatocytes. | en_US |
| dc.description.sponsorship | This work was supported by the National Institutes of Health R35GM128890 to DEL, a Jane Coffin Childs Postdoctoral Fellowship and National Institutes of Health 1K99HD109505-01 to CKC, National Institutes of Health R35GM126964 to AMV, and a Stanford Dean’s Fellowship award to CJU. DEL is also a Searle Scholar and recipient of a March of Dimes Basil O’Connor Starter Scholar award. SIM imaging was performed at Stanford University Cell Sciences Imaging Core Facility, which is supported by Award Number 1S10OD01227601 from the National Center for Research Resources. | en_US |
| dc.identifier.citation | Cahoon, CK; Uebel, CJ; Villeneuve, AM; Libuda, DE (2023). Epitope tag-specific differences in the detection of COSA-1 marked crossover sites in C. elegans spermatocytes. microPublication Biology. 10.17912/micropub.biology.000724. | en_US |
| dc.identifier.uri | https://hdl.handle.net/1794/27913 | |
| dc.identifier.uri | https://doi.org/10.17912/micropub.biology.000724 | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Caltech Library | en_US |
| dc.rights | Creative Commons BY-NC-ND 4.0-US | en_US |
| dc.title | Epitope tag-specific differences in the detection of COSA-1 marked crossover sites in C. elegans spermatocytes | en_US |
| dc.type | Article | en_US |