Lipid‑protein and lipid‑lipid interactions in cytochrome oxidase model membranes
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Date
1973
Authors
Jost, P. C.
Capaldi, R. A.
Vanderkooi, G.
Griffith, O. H.
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Supramolecular Structure
Abstract
Two lipid environments are dete cted in membranous cyto chrome oxidase , using spin
labeling techniques. This model membrane system consists of closed vesicular membranes
formed spontaneou sly when the mem hr,jlne protein is isolated with its accompanying
phospholipids. The data show both an immobili zed componen t, which
is constant in amount , and a fluid componen t. Based on spectral analysis, the interpretation
is that the bound component repre sents a single layer of lipid immobilized
on the surface of the protein between the hydrophobic protein complex and the
adjacent fluid bilayer regions. Maximal enzyme activity of this functional protein
complex is attained when all of the bound sites are occupied , and above this level
additional phospholipid (bilayer) has little or no effect on the enzyme activity .
Description
14 pages
Keywords
Citation
Jost, P. C., Capaldi, R. A., Vanderkooi, G. & Griffith, O. H. (1973) Lipid‑protein and lipid‑lipid interactions in cytochrome oxidase model membranes. J. Supramol. Struc. 1, 269‑280.